Methods
A PCR-based method for detection and quantification of small RNAs.
Ro S, Park C, Jin J, Sanders KM, Yan W.
Biochem Biophys Res Commun. 2006 Dec 22;351(3):756-63.
Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody.
Ikeda K, Satoh M, Pauley KM, Fritzler MJ, Reeves WH, Chan EK.
J Immunol Methods. 2006 Dec 20;317(1-2):38-44.
Absolute quantitation of microRNAs with a PCR-based assay.
Duncan DD, Eshoo M, Esau C, Freier SM, Lollo BA.
Anal Biochem. 2006 Dec 15;359(2):268-70.
Effect of adenovirus-mediated RNA interference on endogenous microRNAs in a mouse model of multidrug resistance protein 2 gene
silencing.
Narvaiza I, Aparicio O, Vera M, Razquin N, Bortolanza S, Prieto J, Fortes P.
J Virol. 2006 Dec;80(24):12236-47.
Design of expression vectors for RNA interference based on miRNAs and RNA splicing.
Du G, Yonekubo J, Zeng Y, Osisami M, Frohman MA.
FEBS J. 2006 Dec;273(23):5421-7.
Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor
plasmid.
De Pietri Tonelli D, Calegari F, Fei JF, Nomura T, Osumi N, Heisenberg CP, Huttner WB.
Biotechniques. 2006 Dec;41(6):727-32.
SAGE detects microRNA precursors.
Ge X, Wu Q, Wang SM.
BMC Genomics. 2006 Nov 7;7:285.
Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions.
Fang S, Lee HJ, Wark AW, Corn RM.
J Am Chem Soc. 2006 Nov 1;128(43):14044-6.
An LNA-based loss-of-function assay for micro-RNAs.
Naguibneva I, Ameyar-Zazoua M, Nonne N, Polesskaya A, Ait-Si-Ali S, Groisman R, Souidi M, Pritchard LL, Harel-Bellan A.
Biomed Pharmacother. 2006 Nov;60(9):633-8.
Vector-based RNAi approaches for stable, inducible and genome-wide screens.
Fewell GD, Schmitt K.
Drug Discov Today. 2006 Nov;11(21-22):975-82.
Optimization of feline immunodeficiency virus vectors for RNA interference.
Harper SQ, Staber PD, Beck CR, Fineberg SK, Stein C, Ochoa D, Davidson BL.
J Virol. 2006 Oct;80(19):9371-80.
Enzymatic microRNA detection in microtiter plates with DNA dendrimers.
Mora JR, Getts RC.
Biotechniques. 2006 Oct;41(4):420, 422, 424.
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression.
Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID.
Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13759-64.
Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides.
Deo M, Yu JY, Chung KH, Tippens M, Turner DL.
Dev Dyn. 2006 Sep;235(9):2538-48.
A microRNA detection system based on padlock probes and rolling circle amplification.
Jonstrup SP, Koch J, Kjems J.
RNA. 2006 Sep;12(9):1747-52.
Lessons from Nature: microRNA-based shRNA libraries.
Chang K, Elledge SJ, Hannon GJ.
Nat Methods. 2006 Sep;3(9):707-14.
A homogenous assay for micro RNA maturation.
Davies BP, Arenz C.
Angew Chem Int Ed Engl. 2006 Aug 18;45(33):5550-2.
Differentiation of 2'-O- and 3'-O-methylated ribonucleosides by tandem mass spectrometry.
Zhang Q, Wang Y.
J Am Soc Mass Spectrom. 2006 Aug;17(8):1096-9.
Pursuing gene regulation 'logic' via RNA interference and chromatin immunoprecipitation.
Li CC, Ramirez-Carrozzi VR, Smale ST.
Nat Immunol. 2006 Jul;7(7):692-7.
Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown.
Sun D, Melegari M, Sridhar S, Rogler CE, Zhu L.
Biotechniques. 2006 Jul;41(1):59-63.
A robust system for RNA interference in the chicken using a modified microRNA operon.
Das RM, Van Hateren NJ, Howell GR, Farrell ER, Bangs FK, Porteous VC, Manning EM, McGrew MJ, Ohyama K, Sacco MA, Halley PA,
Sang HM, Storey KG, Placzek M, Tickle C, Nair VK, Wilson SA.
Dev Biol. 2006 Jun 15;294(2):554-63.
Short hairpin RNA-expressing bacteria elicit RNA interference in mammals.
Xiang S, Fruehauf J, Li CJ.
Nat Biotechnol. 2006 Jun;24(6):697-702.
Strategies to determine the biological function of microRNAs.
Krutzfeldt J, Poy MN, Stoffel M.
Nat Genet. 2006 Jun;38 Suppl:S14-9.
[Genome-wide lentivector-based pooled shRNA library optimization]
Gur'ianova OA, Makhanov M, Chenchik AA, Chumakov PM, Frolova EI.
Mol Biol (Mosk). 2006 May-Jun;40(3):448-59.
Recapitulation of short RNA-directed translational gene silencing in vitro.
Wang B, Love TM, Call ME, Doench JG, Novina CD.
Mol Cell. 2006 May 19;22(4):553-60.
Improved targeting of miRNA with antisense oligonucleotides.
Davis S, Lollo B, Freier S, Esau C.
Nucleic Acids Res. 2006 May 11;34(8):2294-304.
LNA-modified oligonucleotides mediate specific inhibition of microRNA function.
Orom UA, Kauppinen S, Lund AH.
Gene. 2006 May 10;372:137-41.
Microarray tools for deciphering complex diseases.
Shai RM.
Front Biosci. 2006 May 1;11:1414-24.
A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA).
Castoldi M, Schmidt S, Benes V, Noerholm M, Kulozik AE, Hentze MW, Muckenthaler MU.
RNA. 2006 May;12(5):913-20.
Multiplexing RT-PCR for the detection of multiple miRNA species in small samples.
Lao K, Xu NL, Yeung V, Chen C, Livak KJ, Straus NA.
Biochem Biophys Res Commun. 2006 Apr 28;343(1):85-9.
Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155.
Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL.
Nucleic Acids Res. 2006 Apr 13;34(7):e53.
A method for similarity search of genomic positional expression using CAGE.
Seno S, Takenaka Y, Kai C, Kawai J, Carninci P, Hayashizaki Y, Matsuda H.
PLoS Genet. 2006 Apr;2(4):e44.
Detection of microRNAs using electrocatalytic nanoparticle tags.
Gao Z, Yang Z.
Anal Chem. 2006 Mar 1;78(5):1470-7.
Induction of stable RNA interference in mammalian cells.
Cullen BR.
Gene Ther. 2006 Mar;13(6):503-8.
A novel method to monitor the expression of microRNAs.
Fu HJ, Zhu J, Yang M, Zhang ZY, Tie Y, Jiang H, Sun ZX, Zheng XF.
Mol Biotechnol. 2006 Mar;32(3):197-204.
Nonisotopic detection of microRNA using digoxigenin labeled RNA probes.
Ramkissoon SH, Mainwaring LA, Sloand EM, Young NS, Kajigaya S.
Mol Cell Probes. 2006 Feb;20(1):1-4.
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes.
Kloosterman WP, Wienholds E, de Bruijn E, Kauppinen S, Plasterk RH.
Nat Methods. 2006 Jan;3(1):27-9.
A single-molecule method for the quantitation of microRNA gene expression.
Neely LA, Patel S, Garver J, Gallo M, Hackett M, McLaughlin S, Nadel M, Harris J, Gullans S, Rooke J.
Nat Methods. 2006 Jan;3(1):41-6.
Construction of microRNA-containing vectors for expression in mammalian cells.
Fukuda Y, Kawasaki H, Taira K.
Methods Mol Biol. 2006;338:167-73.
Analyzing micro-RNA expression using microarrays.
Davison TS, Johnson CD, Andruss BF.
Methods Enzymol. 2006;411:14-34.
Methodologies for high-throughput expression profiling of microRNAs.
Einat P.
Methods Mol Biol. 2006;342:139-57.
Cloning microRNAs from mammalian tissues.
Michael MZ.
Methods Mol Biol. 2006;342:189-207.
In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates.
Ishizuka A, Saito K, Siomi MC, Siomi H.
Methods Mol Biol. 2006;342:277-86.
Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector.
Luo F, Yee JK, Huang SH, Wu LT, Jong AY.
Methods Mol Biol. 2006;342:287-93.
Gene silencing in vitro and in vivo using intronic microRNAs.
Lin SL, Ying SY.
Methods Mol Biol. 2006;342:295-312.
Isolation and identification of gene-specific microRNAs.
Lin SL, Chang DC, Ying SY.
Methods Mol Biol. 2006;342:313-20.
Transgene-like animal models using intronic microRNAs.
Lin SL, Chang SJ, Ying SY.
Methods Mol Biol. 2006;342:321-34.
MicroRNA protocols. Perspectives.
Ying SY, Chang DC, Miller JD, Lin SL.
Methods Mol Biol. 2006;342:351-8.
Inducible microRNA expression by an all-in-one episomal vector system.
Epanchintsev A, Jung P, Menssen A, Hermeking H.
Nucleic Acids Res. 2006;34(18):e119.
Detection of the mature, but not precursor, RNA using a fluorescent DNA probe.
Paiboonskuwong K, Kato Y.
Nucleic Acids Symp Ser (Oxf). 2006;(50):327-8.
RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts.
Keene JD, Komisarow JM, Friedersdorf MB.
Nat Protoc. 2006;1(1):302-7.
220-plex microRNA expression profile of a single cell.
Tang F, Hajkova P, Barton SC, O'Carroll D, Lee C, Lao K, Surani MA.
Nat Protoc. 2006;1(3):1154-9.
Cell fate simulation model of gustatory neurons with MicroRNAs double-negative feedback loop by hybrid functional Petri net with extension.
Saito A, Nagasaki M, Doi A, Ueno K, Miyano S.
Genome Inform. 2006;17(1):100-11.