Methods
Design of shRNAs for RNAi-A lesson from pre-miRNA processing: possible clinical applications.
Pekarik V.
Brain Res Bull. 2005 Dec 15;68(1-2):115-20.
Silencing of microRNAs in vivo with 'antagomirs'.
Krutzfeldt J, Rajewsky N, Braich R, Rajeev KG, Tuschl T, Manoharan M, Stoffel M.
Nature. 2005 Dec 1;438(7068):685-9.
Real-time quantification of microRNAs by stem-loop RT-PCR.
Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ,
Guegler KJ.
Nucleic Acids Res. 2005 Nov 27;33(20):e179.
Second-generation shRNA libraries covering the mouse and human genomes.
Silva JM, Li MZ, Chang K, Ge W, Golding MC, Rickles RJ, Siolas D, Hu G, Paddison PJ, Schlabach MR, Sheth N, Bradshaw J, Burchard
J, Kulkarni A, Cavet G, Sachidanandam R, McCombie WR, Cleary MA, Elledge SJ, Hannon GJ.
Nat Genet. 2005 Nov;37(11):1281-8.
Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs.
Raymond CK, Roberts BS, Garrett-Engele P, Lim LP, Johnson JM.
RNA. 2005 Nov;11(11):1737-44.
Approaches for chemically synthesized siRNA and vector-mediated RNAi.
Amarzguioui M, Rossi JJ, Kim D.
FEBS Lett. 2005 Oct 31;579(26):5974-81.
Facile means for quantifying microRNA expression by real-time PCR.
Shi R, Chiang VL.
Biotechniques. 2005 Oct;39(4):519-25.
A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells.
Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ.
Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13212-7.
An optimized isolation and labeling platform for accurate microRNA expression profiling.
Shingara J, Keiger K, Shelton J, Laosinchai-Wolf W, Powers P, Conrad R, Brown D, Labourier E.
RNA. 2005 Sep;11(9):1461-70.
RNAi versus small molecules: different mechanisms and specificities can lead to different outcomes.
Fitzgerald K.
Curr Opin Drug Discov Devel. 2005 Sep;8(5):557-66.
Rational probe optimization and enhanced detection strategy for microRNAs using microarrays.
Goff LA, Yang M, Bowers J, Getts RC, Padgett RW, Hart RP.
RNA Biol. 2005 Jul-Sep;2(3):93-100.
An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi.
Zhou H, Xia XG, Xu Z.
Nucleic Acids Res. 2005 Apr 1;33(6):e62.
Inhibition of micro-RNA-induced RNA silencing by 2'-o-methyl oligonucleotides in Drosophila S2 cells.
Berger EM, Dubrovsky EB, Appleby L, Dubrovskaya V.
In Vitro Cell Dev Biol Anim. 2005 Jan-Feb;41(1-2):12-8.
An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe.
Liang RQ, Li W, Li Y, Tan CY, Li JX, Jin YX, Ruan KC.
Nucleic Acids Res. 2005 Jan 31;33(2):e17.
Genome informatics: taming the avalanche of genomic data.
Sonnhammer EL.
Genome Biol. 2005;6(1):301.
Mechanistic insights aid computational short interfering RNA design.
Boese Q, Leake D, Reynolds A, Read S, Scaringe SA, Marshall WS, Khvorova A.
Methods Enzymol. 2005;392:73-96.
Use of RNA polymerase II to transcribe artificial microRNAs.
Zeng Y, Cai X, Cullen BR.
Methods Enzymol. 2005;392:371-80.
Preparation and analysis of Drosha.
Lee Y, Kim VN.
Methods Mol Biol. 2005;309:17-28.
Immunoprecipitation of microRNPs and directional cloning of microRNAs.
Maniataki E, De Planell Saguer MD, Mourelatos Z.
Methods Mol Biol. 2005;309:283-94.
Detection of microRNAs and assays to monitor microRNA activities in vivo and in vitro.
Kiriakidou M, Nelson P, Lamprinaki S, Sharma A, Mourelatos Z.
Methods Mol Biol. 2005;309:295-310.
High-throughput analysis of microRNA gene expression using sensitive probes.
Sioud M, Rosok O.
Methods Mol Biol. 2005;309:311-20.